What is L-Citrulline Based Amino Blend (20ML)?

L-Citrulline Based Amino Blend (20ML) is a nine-component research solution of L-citrulline, L-arginine hydrochloride, L-alanyl-L-glutamine, taurine, L-lysine hydrochloride, L-proline, L-carnitine, N-acetylcysteine and lidocaine. L-carnitine (150 mg/mL), not L-citrulline (85 mg/mL), is the largest component by mass, and the glutamine source is the dipeptide L-alanyl-L-glutamine rather than free L-glutamine.

L-citrulline and L-arginine form the recycling loop of nitric oxide synthesis: nitric oxide synthases make L-citrulline from L-arginine (Förstermann & Sessa, 2012), and argininosuccinate synthetase and lyase convert it back (Wu & Morris, 1998). L-carnitine adds the CPT acyl shuttle (McGarry & Brown, 1997), and N-acetylcysteine is a cysteine and glutathione precursor (Pedre et al., 2021).

Batch-verified at Modern Aminos, it is supplied in one laboratory format, a 20 mL vial. The carrier is a water-based solution (RO water carrier) with benzyl alcohol (1%) as the preservative. Per-mL composition:

• L-Arginine HCl: 75 mg/mL
• L-Citrulline: 85 mg/mL
• L-Alanyl-L-Glutamine (Ala-Gln): 44.6 mg/mL
• Taurine: 45 mg/mL
• L-Lysine HCl: 35 mg/mL
• L-Proline: 30 mg/mL
• L-Carnitine: 150 mg/mL
• N-Acetylcysteine (NAC): 40 mg/mL
• Lidocaine: 10 mg/mL

Laboratories building metabolic panels often place it next to public reference listings such as DADA (Diisopropylamine Dichloroacetate).

Chemical and Molecular Data for L-Citrulline Based Amino Blend (20ML)

Each component is identified below from its PubChem record; amounts are per mL of finished solution, and salt forms are listed as weighed.

L-Arginine HCl (75 mg/mL)

L-Citrulline (85 mg/mL)

L-Alanyl-L-Glutamine (Ala-Gln) (44.6 mg/mL)

Taurine (45 mg/mL)

L-Lysine HCl (35 mg/mL)

L-Proline (30 mg/mL)

L-Carnitine (150 mg/mL)

N-Acetylcysteine (NAC) (40 mg/mL)

Lidocaine (10 mg/mL)

What are the Mechanisms of Action?

The pathway chemistry behind L-Citrulline Based Amino Blend (20ML) in laboratory research includes:

• Citrulline recycling: L-citrulline, the co-product of nitric oxide synthase, is converted back to L-arginine by argininosuccinate synthetase and argininosuccinate lyase (Wu & Morris, 1998).
• Arginine branch point: L-arginine is the substrate of the nitric oxide synthases, which oxidize its guanidino nitrogen to nitric oxide and L-citrulline using NADPH, O₂ and tetrahydrobiopterin (Förstermann & Sessa, 2012). Arginase competes for the same pool, hydrolyzing L-arginine to L-ornithine and urea (Wu & Morris, 1998).
• Carnitine shuttle: L-carnitine accepts long-chain acyl groups from acyl-CoA through carnitine palmitoyltransferase 1 (CPT1) at the outer mitochondrial membrane; the acylcarnitine crosses the inner membrane, and CPT2 regenerates acyl-CoA in the matrix, with malonyl-CoA inhibition of CPT1 as the control point (McGarry & Brown, 1997). Endogenous L-carnitine is built from 6-N-trimethyllysine released from methylated proteins (Vaz & Wanders, 2002).
• Thiol donor: N-acetylcysteine is deacetylated to L-cysteine, the rate-limiting precursor of glutathione, and cysteine-derived hydrogen sulfide and sulfane sulfur species have been proposed as part of its antioxidant chemistry (Pedre et al., 2021).
• Glutamine dipeptide: L-alanyl-L-glutamine is a dipeptide of L-alanine and L-glutamine (ChEBI); the free glutamine it carries is the substrate of glutaminase, which hydrolyzes it to glutamate and ammonia (Cruzat et al., 2018).
• Taurine chemistry: Taurine (2-aminoethanesulfonic acid) carries a sulfonic acid group instead of a carboxyl group; it is not incorporated into proteins and is conjugated to bile acids (Ripps & Shen, 2012).

What Does Published Research Show for L-Citrulline Blend?

Published reviews on the components of L-Citrulline Based Amino Blend (20ML) center on their pathway biochemistry:

• Arginine Metabolism (Review, PMC): Wu and Morris mapped the enzymes that use L-arginine, including NOS, arginase and the citrulline-recycling enzymes (Wu & Morris, 1998).
• Nitric Oxide Synthases (Review, PMC): Förstermann and Sessa reviewed the three NOS isoforms, their cofactors and their regulation (Förstermann & Sessa, 2012).
• Carnitine Palmitoyltransferase System (Review): McGarry and Brown traced the CPT system from the original carnitine-shuttle concept to the molecular analysis of CPT1 and CPT2, including malonyl-CoA control of CPT1 (McGarry & Brown, 1997).
• Carnitine Biosynthesis (Review, PMC): Vaz and Wanders described the four enzymatic steps from 6-N-trimethyllysine to L-carnitine, ending at γ-butyrobetaine hydroxylase (Vaz & Wanders, 2002).
• N-Acetylcysteine Mechanism (Review): Pedre and colleagues reviewed NAC chemistry, from cysteine and glutathione supply to hydrogen sulfide and sulfane sulfur species (Pedre et al., 2021).
• Glutamine Metabolism (Review, PMC): Cruzat and colleagues reviewed glutamine synthesis by glutamine synthetase and hydrolysis by glutaminase (Cruzat et al., 2018).
• Taurine Biochemistry (Review, PMC): Ripps and Shen reviewed taurine’s synthesis, transport and roles as a free amino sulfonic acid (Ripps & Shen, 2012).
• Chemical Directory & Structure Verification (PubChem): L-Arginine CID 66250; L-Citrulline CID 9750; Ala-Gln CID 123935; Taurine CID 1123; L-Lysine CID 69568; L-Proline CID 145742; L-Carnitine CID 10917; NAC CID 12035; Lidocaine CID 3676. Identifiers on this page were checked against these records on October 8, 2026.
• Analytical Quality: Why chromatographic testing matters for reference materials is covered in Modern Aminos’ note on HPLC testing.

How does L-Citrulline Blend compare to other compounds?

To place L-Citrulline Blend among related research materials, the table compares it with other solutions and references available at Modern Aminos:

Frequently Asked Questions (FAQs)