Modern Amino’s high-quality, USA-made Vitamin B-12 is third-party tested for accurate dosing to guarantee potency and strength.
SOLD FOR RESEARCH PURPOSES ONLY
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Vitamin B12 (Methylcobalamin) is a 20 mL research solution of methylcobalamin, the methyl form of vitamin B12 in which a methyl group is the upper axial ligand on the cobalt of the corrin ring. This product is methylcobalamin, not cyanocobalamin: the upper axial ligand on cobalt is a methyl group rather than a cyano group, and no cyanocobalamin is listed in the formula.
Methylcobalamin is the cofactor of cobalamin-dependent methionine synthase, which transfers a methyl group from 5-methyltetrahydrofolate to homocysteine to form methionine (Banerjee & Matthews, 1990). The reaction links the folate and methionine cycles (Froese et al., 2019).
Batch-verified at Modern Aminos, it is supplied in one laboratory format, a 20 mL vial. The carrier is a water-based solution (RO water carrier) with benzyl alcohol (1%) as the preservative. Per-mL composition:
Laboratories building metabolic panels often place it next to public reference listings such as DADA (Diisopropylamine Dichloroacetate).
| Compound Name | Vitamin B12 (Methylcobalamin) |
|---|---|
| Research Focus | Neurodegenerative, Neurogenesis, Methylation Pathways, Hematology, Erythropoiesis, Megaloblastic Anemia, DNA Synthesis, intracellular oxidative nitrosative, Epigenetic, DNA Methylation, Methionine Synthase, Hyperhomocysteinemia |
| Quantity / Formats Offered | 2.5 mg/mL (20 mL Vial) |
| Synonyms / Alt Names | Vitamin B12, Methylcobalamin), mecobalamin, methyl B12, B12 |
| PubChem CID | 10898559 |
| CAS Number | 13422-55-4 |
| ChEBI | CHEBI:28115 |
| Chemical Formula | C63H91CoN13O14P |
| Molecular Weight | 1344.4 g/mol |
| Exact Mass | 1343.5878 Da |
| IUPAC Name | carbanide;cobalt(3+);[(2R,3S,4R,5S)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] 1-[3-[(1R,2R,3R,5Z,7S,10Z,12S,13S,15Z,17S,18S,19R)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7,12,17-tetrahydro-1H-corrin-24-id-3-yl]propanoylamino]propan-2-yl phosphate |
| InChIKey | ZFLASALABLFSNM-WYVZQNDMSA-L |
| Carrier / Excipients | Aqueous solution containing sterile water with 1% benzyl alcohol (preservative) |
The pathway chemistry behind Vitamin B12 in laboratory research includes:
| Cobalamin form | Upper axial ligand on cobalt | Enzyme context | In this product? |
|---|---|---|---|
| Methylcobalamin | Methyl (–CH3) | Methionine synthase cofactor (Banerjee & Matthews, 1990) | Yes, 2.5 mg/mL |
| Adenosylcobalamin | 5′-Deoxyadenosyl | Methylmalonyl-CoA mutase cofactor (Froese et al., 2019) | No |
| Cyanocobalamin | Cyano (–CN) | Not a cofactor form; must be converted before use by cobalamin enzymes | No |
Published reviews on the components of Methylcobalamin center on their pathway biochemistry:
To place Vitamin B12 among related research materials, the table compares it with other solutions and references available at Modern Aminos:
| Compound / Reference | Primary Target Class | Core Mechanism of Action | Research Focus & Profile |
|---|---|---|---|
| Vitamin B12 (Methylcobalamin) | Cobalamin cofactor | Methyl donor to homocysteine via methionine synthase | One-carbon and remethylation panels |
| ATP/AMP/NALT Based Amino Blend | Nucleotides + amino acids | Includes betaine (TMG), a methyl donor through BHMT | Methyl-donor comparison |
| DADA (Diisopropylamine Dichloroacetate) | PDK inhibitor | Single defined small molecule | Contrast: enzyme inhibitor vs cofactor |
Vitamin B12 (20ML) is available at Modern Aminos as a 20 mL research solution (SKU A-001-F). If you want to buy Vitamin B12 (Methylcobalamin) as a documented reference material, each certificate is in the Certificate of Analysis tab.
Per the Modern Aminos manufacturing formula (SKU A-001-F), each mL contains Methylcobalamin 2.5 mg/mL (2,500 mcg/mL). Carrier: water-based solution (RO water carrier) with benzyl alcohol (1%) as the preservative.
No. Methylcobalamin is a cobalt-containing corrinoid, a form of vitamin B12, and contains no peptide bonds. It is supplied as a research solution, not as a supplement.
PubChem lists Methylcobalamin as CID 10898559 with CAS 13422-55-4, formula C63H91CoN13O14P, molecular weight 1344.4 g/mol and InChIKey ZFLASALABLFSNM-WYVZQNDMSA-L; ChEBI lists it as CHEBI:28115.
Methylcobalamin (PubChem CID 10898559, CAS 13422-55-4, ChEBI:28115). The manufacturing formula and the label both specify methylcobalamin, and the raw-lot certificate cites the same PubChem record. Cyanocobalamin carries a cyano group in place of the methyl group and is not part of this product.
The formula lists methylcobalamin at 2.5 mg/mL, which is 2,500 mcg/mL, matching the product’s form attribute “Methylcobalamin 2500MCG/ML.”
Cobalamin-dependent methionine synthase, which carries the methyl group from 5-methyltetrahydrofolate to homocysteine via the cobalt center to form methionine (Banerjee & Matthews, 1990; Froese et al., 2019).
PubChem lists C63H91CoN13O14P and 1344.4 g/mol (exact mass 1343.5878 Da) for CID 10898559, consistent with the 1343.59 Da expected mass printed on the raw-lot certificate.
Cobalamins share the corrin ring and cobalt center and differ in the upper axial ligand: methyl in methylcobalamin, cyano in cyanocobalamin and 5′-deoxyadenosyl in adenosylcobalamin. Methylcobalamin is the form used by methionine synthase.
One format: a 20 mL vial of solution at the per-mL amounts listed above. The vial label lists the per-mL ingredients, and the Certificate of Analysis tab lists the certificates for the current format.
The MZ Biolabs certificate for methylcobalamin lot 2026-01-29 lists an HPLC-UV purity of 99.18% (main peak at 5.77 min) and HPLC-MS identity by monoisotopic mass (1343.65 Da measured, 1343.59 Da expected). The finished 20 mL format lists a USP <71> sterility pass from Vanguard Laboratory (5/29/2026). For background on chromatographic testing, see why HPLC testing matters.
Keep the sealed vial cool, dry and away from light, and follow the label on each lot. No storage temperature is printed on the label or the certificates, so this page does not state one.
DISCLAIMER: All compounds and research materials supplied by Modern Aminos are intended exclusively for in vitro laboratory research by qualified professionals. They are not medicinal products and have not been authorised by the European Medicines Agency or any national competent authority for human or veterinary use. They are not foods, food supplements, cosmetics or medical devices. Products must not be administered to humans or animals by any route and must not be used for in vivo experimentation or any other non-laboratory purpose. We will refuse or cancel orders, and may close accounts, where there is any indication of human or animal use. By purchasing, you confirm that products will be used only in a controlled laboratory environment by appropriately qualified personnel, in line with all applicable laws.
All products are sold in powder (lyophilized) form and require reconstitution with a suitable diluent for research purposes only. Research supplies (e.g., syringes, bacteriostatic water) are not included. No dosing instructions are provided. We adhere to all local and state laws around Research Only Chemical sales. We are not a pharmacy, nor do we promote or provide any advice for human or animal consumption. Please review our terms and conditions carefully before making a purchase on our website.
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